Extraction of arbuscular mycorrhizal fungus spores from clayey soil
نویسندگان
چکیده
منابع مشابه
Identification and isolation of two ascomycete fungi from spores of the arbuscular mycorrhizal fungus Scutellospora castanea.
Two filamentous fungi with different phenotypes were isolated from crushed healthy spores or perforated dead spores of the arbuscular mycorrhizal fungus (AMF) Scutellospora castanea. Based on comparative sequence analysis of 5.8S ribosomal DNA and internal transcribed spacer fragments, one isolate, obtained from perforated dead spores only, was assigned to the genus Nectria, and the second, obt...
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The aqueous biphasic system (ABS) method using PEG and ammonium sulphate improved the recovery of spores from tropical soils as compared with the sucrose gradient method. The ABS was not affected by low temperatures. The optimal recovery of spores was achieved when 100% PEG and 50% ammonium sulphate concentration and PEG8000 instead of PEG4000 were used. Under these conditions an optimal interf...
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This study was aimed to investigate the diversity and function of bacterial population associated with Gigaspora margarita spores. The fungus was propagated in sterilized sand/soil pots using alfalfa (Medicago sativa), grain sorghum (Sorghum bicolor), or maize (Zea mays) as host plants, or in sterilized vermiculite pots using alfalfa as host plants, respectively. Bacteria were isolated from the...
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As Neotropical forests are increasingly converted to agriculture (especially pastures), little is known about the impacts on microbial biodiversity. To assess such impacts, I compared spore abundance and diversity of arbuscular mycorrhizal fungi (AMF) in soils from lowland evergreen forests and pastures in Nicaragua and Costa Rica. Species composition, dominance-diversity curves, and Simpson’s ...
متن کاملMolecular Identification of Arbuscular Mycorrhizal Fungal Spores Collected in Korea
Arbuscular mycorrhizas (AM) have mutualistic symbiosis with plants and thus efforts have been placed on application of these symbiotic relationships to agricultural and environmental fields. In this study, AM fungi were collected from 25 sites growing with 16 host plant species in Korea and cultured with Sorghum bicolor in greenhouse condition. AM fungal spores were extracted and identified usi...
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ژورنال
عنوان ژورنال: Tropics
سال: 2007
ISSN: 0917-415X,1882-5729
DOI: 10.3759/tropics.16.337